Open AccessIsolation of Agrobacterium sp., strain from the Azolla leaf cavity
Author(s) -
Plazinski Jacek,
Taylor Rona,
Shaw William,
Croft Lynn,
Rolfe Barry G.,
Gunning Brian E.S.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03777.x
Subject(s) - agrobacterium tumefaciens , biology , strain (injury) , plasmid , microbiology and biotechnology , dna , gene , genetics , transformation (genetics) , anatomy
We have isolated a bacterial strain from surface sterilized fronds of the aquatic fern Azolla filiculoides and infer that it occurs in leaf cavities, along with the nitrogen‐fixing cyanobacterium Anabaena azollae . This strain grew slowly on rich media, appeared to be Gram‐negative, and was identified using a bacteriological multitest system as Agrobacterium sp. This strain, designated AFSR‐1, could grow in the presence of 400 ppm of ammonium sulphate. DNA/DNA hydrodization analysis showed that a 16 S ribosomal RNA gene is located in the AFSR‐1 strain on an identical DNA restriction fragment as in a strain of Agrobacterium tumefaciens . In addition, it was found that strain AFSR‐1 contained DNA regions homologous to the modulation genes nodABC and nodL of Rhizobium trifolii , AFSR‐1 contains a cryptic plasmid, and the genetic transfer of a broad‐host‐range plasmid pLAFR3 to strain AFSR‐1 was achieved.