Open AccessProstaglandin E 2 production by rat peritoneal macrophages: role of cellular and humoral factors in vivo in transfusion‐associated immunosuppression
Author(s) -
Ross W.B,
Leaver H.A.,
Yap P.L.,
Raab G.M.,
Su B.H.,
Carter D.C.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03535.x
Subject(s) - immunosuppression , mononuclear phagocyte system , immunology , macrophage , peripheral blood mononuclear cell , immunosurveillance , phagocyte , blood transfusion , biology , in vivo , whole blood , blood cell , medicine , immune system , in vitro , biochemistry , microbiology and biotechnology
The transfusion of blood is associated with long‐term immunosuppression, which has been postulated to influence immunosurveillance and cancer cell killing. The mononuclear phagocyte synthesises large quantities of PGE 2 , and PGE 2 has been shown to inhibit the activity of a range of immunocompetent cell types. The role of mononuclear phagocyte PGE 2 synthesis in transfusion‐associated immunosuppression, and the elements of transfused blood which control this immunosuppression, were investigated using a transfused rat model. A significant increase in macrophage PGE 2 synthesis was detected 7 days after transfusion with blood and serum. The storage of blood for 24 h increased the stimulatory activity of transfused blood. The effects of storage and serum on macrophage PGE 2 synthesis were greater than effects due to genetic differences between blood donor and recipient, and the serum effects indicated that a major factor activating PGE 2 ‐mediated immunosuppression in transfused subjects may be humoral in nature.