Open AccessThe regulatory role of external cations on human polymorphonuclear phagocyte chemiluminescence
Author(s) -
Janah S.,
Mali B.,
Fleck A.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03534.x
Subject(s) - chemiluminescence , chemistry , phagocyte , biophysics , amiloride , antiporter , granulocyte , respiratory burst , biochemistry , chromatography , phagocytosis , sodium , immunology , membrane , biology , organic chemistry
The effect of the external cations Na + and Ca 2+ on polymorphonuclear chemiluminescence was investigated. Both Ca 2+ in the range of 0.2–2 mM and Na + in the range of 114–143 mM showed a dose dependent increase in polymorphonuclear chemiluminescence, irrespective of the concurrent increase in osmolality. The Na + /H + antiport inhibitor Amiloride decreased the response significantly. These effects were observed using buffers commonly used for chemiluminescence studies and indicate the importance of defining the Ca 2+ and Na + composition of the buffers used in chemiluminescence assays.