Open AccessThe effect of endotoxin lipopolysaccharide from different bacterial species on the generation of intracellular inositol triphosphate and superoxide in a human phagocytic cell line
Author(s) -
Jiamin Qu,
Anne Leaver H.,
Aldhous Marian C.,
Lee Yap Peng
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03530.x
Subject(s) - lipopolysaccharide , superoxide , intracellular , inositol , chemistry , microbiology and biotechnology , biology , biochemistry , immunology , receptor , enzyme
The acute effects of endotoxins and lipid A on two intracellular responses, inositol phosphate generation and superoxide production were analysed in the DMSO differentiated premyelocytic leukamic HL‐60 cell line. Short‐term incubation (1–10 min) with Escheria coli ‐type LPS, Salmonella ‐type LPS and Lipid A caused significant increases in cellular InsP 1 and InsP 3 , compared with control cells ( P < 0.5 − P < 0.001). The Escheria coli ‐type B LPS released approximately twice the quantity of InsP 3 compared with Salmonella ‐type LPS ( P < 0.001). Lipid A‐dependent stimulation of InsP 3 production was also detected. The rate of superoxide production increased 1–10 min after addition of both Escheria coli ‐ and Salmonella ‐type LPS and Lipid A. Endotoxins and Lipid A caused a dose‐dependent increase in intracellular oxidative activity. The superoxide response showed less species dependence and a higher response to particulate lipid A compared with the inositol phosphate response.