Open AccessThe susceptibility of in vivo‐grown Pasteurella haemolytica to ovine defence mechanisms in vitro
Author(s) -
Sutherland A.D.,
Jones G.E.,
Poxton I.R.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03529.x
Subject(s) - in vivo , microbiology and biotechnology , in vitro , biology , immune system , antibody , bacteria , pasteurella , defence mechanisms , immunology , biochemistry , genetics , gene
Pasteurella haemolytica organisms grown in vivo were examined for their susceptibility to ovine immune mechanisms in vitro. Compared with in vitro grown organisms they were less susceptible to opsonophagocytosis and, in contrast, susceptible to complement‐dependent killing in the absence of exogenous antibody. These differences were not associated with phenotypic changes in the surface of the bacterial cell. However, overproduction and de novo synthesis of proteins was observed in in vivo grown organisms. Also, bound host‐immunoglobulin was observed on in vivo grown organisms and a role for this in modifying the interaction with immune mechanisms is discussed.