Open AccessRecognition of 29 kDa surface‐associated adhesive molecule of Entamoeba histolytica by monoclonal antibodies
Author(s) -
Vinayak V.K.,
Shandil R.K.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03516.x
Subject(s) - entamoeba histolytica , monoclonal antibody , biology , chinese hamster ovary cell , microbiology and biotechnology , isotype , clone (java method) , antibody , immunofluorescence , protozoa , antigen , baby hamster kidney cell , biochemistry , cell , receptor , immunology , dna
Monoclonal antibodies have been developed and used as specific probe to locate and identify a 29‐kDa molecule of axenic Entamoeba histolytica trophozoites. Monoclonal antibody produced by clone C8 (MoAb C8) strongly agglutinated the amoebic trophozoites. THe immunofluorescence of live E. histolytica trophozoites and surface fluorescence of acetone‐fixed trophozoites by MoAb C8 indicated existence of a 29‐kDa molecule on surface‐associated plasma membrane of E. histolytica . The monoclonal antibody belonged to IgG 1 isotype. The prior treatment of E. histolytica trophozoites with MoAb C8 resulted in significant ( P < 0.01) reduction in adherence of amoebic trophozoites to cultured Chinese Hamster Ovary cells and significant ( P < 0.01) reduction in cytotoxicity to cultured Baby Hamster Kidney cells. Pretreatment of amoebic trophozoites with MoAb C8 prior to cultivation in TPS‐1 medium resulted in significant ( P < 0.01) reduction in growth of the parasite. Thus, the data suggested that the surface‐exposed 29‐kDa molecule may be one of the receptors involved in E. histolytica host cell interactions and may possibly modulate amoebic disease processes.