Open AccessSpecific inhibition of phagosome‐lysosome fusion in murine macrophages mediated by Salmonella typhimurium infection
Author(s) -
Ishibashi Yoshio,
Arai Toshihiko
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03476.x
Subject(s) - phagosome , lysosome , microbiology and biotechnology , salmonella , biology , phagolysosome , phagocytosis , chemistry , bacteria , biochemistry , enzyme , genetics
Phagosome‐lysosome fusion in murine macrophages infected with S. typhimurium LT2 or S. typhi 1079 was investigated. Fusion of phagosome containing S. typhimurium LT2 with lysosome was markedly impaired, whereas S. typhi 1079 did not inhibit phagosome‐lysosome fusion in murine macrophages. A similar inhibition of fusion was observed with LPS‐deficient mutants of S. typhimurium LT2, suggesting that O‐antigens do not contribute to the inhibition of fusion. Phagosome‐lysosome fusion in macrophages after ingestion of UV‐killed S. typhimurium LT2 was much greater than that of live bacteria. Furthermore, treatment of S. typhimurium LT2 with streptomycin, an inhibitor of bacterial protein synthesis, caused an increase in the extent of phagosome‐lysosome fusion. Therefore protein synthesis in live bacteria is probably required for the inhibition of phagosome‐lysosome fusion. These results suggest that phagosome‐lysosome fusion in murine macrophages is impaired by some product(s) of viable S. typhimurium LT2.