Open AccessBlastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipoolysaccharide, lipid A and acid‐degrade polysaccharide from Fusobacterium nucleatum
Author(s) -
Sveen Kjell,
Hofstad Tor
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03475.x
Subject(s) - spleen , polyclonal antibodies , lipopolysaccharide , fusobacterium nucleatum , antibody , thymidine , lipid a , polysaccharide , biology , incubation , microbiology and biotechnology , biochemistry , chemistry , in vitro , immunology , bacteria , genetics , porphyromonas gingivalis
Lipopolysaccharide of Fusobacterium nucleatum strain Fevl was split by acid hydrolysis. The split products, i.e. lipid A and degraded polysaccharide were mitogenic for murine spleen cells as measured by uptake of [ 3 H]thymidine. The uptake of [ 3 H]thymidine was dose‐dependent. Incubation of spleen cells with stimulants for 3 days resulted in a polyclonal activation of immunoglobulin synthesis. Higher mitogenic response and immunoglobulin production were found in spleen cells of athymic mice compared to those of thymic mice. The activity of lipid A in stimulating immunoglobulin synthesis was comparable with the parent lipopolysaccharide‐Fevl, the degraded polysaccharide being the less potent stimulator.