Open AccessCharacterization of a methyl‐specific restriction system in Clostridium acetobutylicum strain N1‐4081
Author(s) -
Azeddoug H.,
Hubert J.,
Reysset G.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03682.x
Subject(s) - isoschizomer , clostridium acetobutylicum , restriction enzyme , strain (injury) , methylation , clostridium , biology , chemistry , microbiology and biotechnology , dna , genetics , biochemistry , bacteria , gene , dna methylation , butanol , gene expression , anatomy , ethanol
A type II restriction endonuclease, named Cac I, was detected in Clostridium acetobutylicum strain N1‐4081. Cac I cleaved the tetranucleotide sequence [5′↓‐GATC‐3′]. The modification system consisted of the methylation of the adenine present in this sequence. Cac I, an isoschizomer of Mbo I, is inactive on dam methylated substrates.