Open AccessFurther characterization of the photoreaction center from Ectothiorhodospira sp. detection of the H subunit by monoclonal antibodies
Author(s) -
Lefebvre Suzie,
Picorel Rafael,
Gingras Gabriel
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03668.x
Subject(s) - rhodospirillum rubrum , protein subunit , photosynthetic reaction centre , rhodospirillales , microbiology and biotechnology , heme , monoclonal antibody , biology , rhodospirillaceae , hemeprotein , chromatophore , chemistry , antibody , biochemistry , enzyme , photosynthesis , gene , immunology , fishery
The photoreaction center isolated from Ectothiorhodospira sp. is known to contain polypeptides L,M and C (a four heme c cytochrome) in a 1:1:1 molar ratio. As a test for the presence of a H subunit, Western blots of the chromatophore and photoreaction center polypeptides were reacted with a monoclonal antibody directed against the H subunit of Rhodospirillum rubrum . The chromatophores but not the isolated photoreaction center cross‐reacted with this antibody. It is concluded that the H polypeptide is lost during the purification of the photoreaction center which, in its native state, therefore contains four polypeptides including a genuine H subunit.