Open AccessNucleotide sequence of the gene encoding the active‐site serine β‐lactamase from Actinomadura R39
Author(s) -
Houba Simone,
Willem Sabine,
Duez Colette,
Molitor Chantal,
Dusart Jean,
Frère JeanMarie,
Ghuysen JeanMarie
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03667.x
Subject(s) - nucleic acid sequence , serine , biology , peptide sequence , bacillus licheniformis , signal peptide , streptomyces , streptomyces albus , biochemistry , streptomycetaceae , actinomycetales , gene , amino acid , microbiology and biotechnology , bacteria , bacillus subtilis , genetics , enzyme
The gene encoding the extracellular, active‐site serine β‐lactamase of Actinomadura R39, previously cloned into Strptomyces lividans , has the information for the synthesis of a 304 amino acid protein, the amino terminal region of which has the characteristic features of a signal peptide. The Actinomadura R39 β‐lactamase is another member of the class A β‐lactamases. In particular, it shows high homology with the β‐lactamase of Bacillus licheniformis .