Open AccessMolecular cloning of aromatic degradative genes from Pseudomonas stutzeri
Author(s) -
GarcíaValdés E.,
Cózar E.,
Lalucat J.,
Rotger R.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03641.x
Subject(s) - pseudomonas stutzeri , plasmid , molecular cloning , microbiology and biotechnology , gene , catechol , dna , biology , restriction map , cloning (programming) , homology (biology) , pseudomonas , chemistry , biochemistry , complementary dna , genetics , bacteria , computer science , programming language
A 6.1 kilobase DNA fragment has been cloned from Pseudomonas stutzeri AN11, a naphthalene‐ degrading marine isolate. The cloned DNA constitutively expressed salicylate hydroxylase and catechol‐2,3‐dioxygenase activities in E. coli , both activities being slightly induced by salicylate. The structural genes have been located in the cloned fragment, which hybridized both with the chromosomal DNA and with a 60 kilobase plasmid of P. stutzeri AN11, and showed homology with the corresponding regions of TOL and NAH plasmids.