Open AccessBacterial colony screening with a Streptomyces DNA probe
Author(s) -
Kuczek K.,
Mordarski M.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03633.x
Subject(s) - streptomyces , streptomycetaceae , biology , actinomycetales , dna , hybridization probe , microbiology and biotechnology , molecular probe , nucleic acid thermodynamics , dna–dna hybridization , bacteria , genetics , base sequence
Restriction fragments of 1.5 kb–3.5 kb length were selected from a Sal I digest of Streptomyces coriofaciens ISP5485 DNA. After radiolabelling, these fragments were used as a molecular probe. A number of actinomycetes was screened in colony hybridization. Streptomyces and Streptoverticillium strains were recognized by the probe and the hybridization sensitivity was high with isolated DNA.