Open AccessCuring of the killer character of Saccharomyces cerevisiae with acridine orange
Author(s) -
Cansado José,
Longo Elisa,
Agrelo Dolores,
Villa Tomás G.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03628.x
Subject(s) - curing (chemistry) , acridine orange , plasmid , orange (colour) , microbiology and biotechnology , chemistry , biology , food science , biochemistry , dna , polymer chemistry , apoptosis
Acridine orange, an intercalating dye usually employed in the curing of bacterial plasmids, was tested for its ability to cure K 1 and K 2 killer strains (laboratory and wine strains). The results showed a high curing percentage of the killer character. This was demonstrated by the loss of M 1 or M 2 dsRNAs (responsible for toxin production and resistance to it) and because the meiotic products exhibited non‐Mendelian segregation. The curing percentages varied, depending on the strain but not on the killer type, and showed similar efficiency as compared with other known curing agents.