Open AccessIntroduction of the transposon Tn919 into Lactobacillus curvatus Lc2‐c
Author(s) -
Knauf Hermann J.,
Vogel Rudi F.,
Hammes Walter P.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03604.x
Subject(s) - transposable element , transposition (logic) , enterococcus faecalis , plasmid , microbiology and biotechnology , strain (injury) , biology , enterococcus , lactobacillus , genetics , escherichia coli , dna , bacteria , mutant , gene , anatomy , computer science , artificial intelligence
The conjugative transposon Tn 919 was introduced into the plasmid cured strain Lactobacillus curvatus Lc 2‐c by means of filter mating. Enterococcus faecalis GF590 was used as donor. The transfer frequency was 2 × 10 −7 /recipient. Random insertion was proved by Southern hybridization. Segregation after 10 passages without selective pressure could not be detected. The transposition site remained unchanged and no subsequent second‐site transposition occured.