Open AccessCell wall constituents of Microcystis sp. PCC 7806
Author(s) -
Jürgens Uwe J.,
Martin Cornel,
Weckesser Jürgen
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03595.x
Subject(s) - peptidoglycan , cell wall , biochemistry , phospholipid , polysaccharide , microcystis aeruginosa , lipopolysaccharide , biology , algae , chemistry , muramic acid , microcystis , glycan , cell envelope , microbiology and biotechnology , cyanobacteria , bacteria , membrane , glycoprotein , botany , escherichia coli , gene , endocrinology , genetics
Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X‐100 extraction. The outer membrane contained carotenoids, two major peptidoglycan‐associated proteins ( M r 49 000 and 52 000), and lipopolysaccharide (LPS) as indicated by the presence of 3‐hydroxy fatty acids (3‐OH‐14: 0, 3‐OH‐16: 0, 3‐OH‐18: 0), 4‐oxo‐18: 0 fatty acid, and GlcN as lipid A components in addition to rare O ‐methyl sugars (2‐ O ‐methyl‐6‐deoxyhexoses I and II). The peptidoglycan (A1γ‐type) was found to be covalently linked to a wall polysaccharide composed of GlcN, ManN, Man, Glc, and phosphate.