Open AccessRecognition of Fusobacterium nucleatum subgroups Fn‐1, Fn‐2 and Fn‐3 by ribosomal RNA gene restriction patterns
Author(s) -
Lawson Paul A.,
Gharbia Saheer E.,
Shah Haroun N.,
Clark Duncan R.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03594.x
Subject(s) - fusobacterium nucleatum , ecori , restriction enzyme , taqi , biology , microbiology and biotechnology , hindiii , ribosomal rna , gene , genetics , dna , restriction fragment length polymorphism , polymerase chain reaction , bacteria , porphyromonas gingivalis
DNA from representative strains of Fusobacterium nucleatum subgroups Fn‐1, Fn‐2 and Fn‐3 was digested with restriction enzymes Eco RI and Taq I and the electrophoretically separated fragmetns hybridized with a 32 P‐16S rRNA gene probe from E. coli . The rRNA gene restriction patterns from DNA digested with either enzyme allowed the clustering of strains into the three subgroups. However, Taq I digested DNA yielded a wider distribution of taxonomically useful bands (ca 0.65 ± 14.3 kbp) and the pattern produced was characteristic of each subgroup. The present method is a simple and reliable means of identifying the three subgroups of F. nucleatum and provides a useful method for further studies of the heterogeneity of F. nucleatum .