Open AccessContent, distribution and stability of protein‐synthesis elongation factor Tu in subcellular fractions of vegetative cells and spores of Streptomyces aureofaciens
Author(s) -
Weiser J.,
Morava P.,
Vohradský J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03589.x
Subject(s) - spore , streptomyces aureofaciens , protease , elongation , biology , elongation factor , ribosome , protein biosynthesis , streptomyces , biochemistry , microbiology and biotechnology , bacteria , rna , enzyme , gene , genetics , materials science , ultimate tensile strength , metallurgy
The protein synthesis elongation factor Tu (EF‐Tu) was identified in dormant spores of Streptomyces aureofaciens and its content and distribution in vegetative cells and dormant spores were determined. Cell‐free homogenates from spores were found to contain a EF‐Tu cleaving membrane bound protease. The protease cleaved aggregated EF‐Tu much less efficiently than non‐aggregated factor in cell homogenates. The relative content of EF‐Tu and ribosomes in dormant spores was very similar to that found in exponentially growing vegetative cells.