Open AccessElectroporation of Azospirillum brasilense with plasmid DNA
Author(s) -
Vande Broek Ann,
Gool August,
Vanderleyden Jos
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03574.x
Subject(s) - electroporation , azospirillum brasilense , plasmid , transformation (genetics) , strain (injury) , microbiology and biotechnology , bacteria , dna , biology , gel electrophoresis , microbial inoculant , genetics , gene , anatomy
The feasibility of electric field mediated transformation of the nitrogen fixing bacterium Azospirillum was studied. The broad host range plasmid pRK290 was used throughout this study. Transformants were obtained with all A. brasilense strains tested, although with strain dependent efficiency. No transformants were obtained with an A. lipoferum strain. Transfer of the pRK290 plasmid DNA in the A. brasilense strains was confirmed by DNA extraction of the transformants and gel electrophoresis. The effects of the physiological status of the cells and the electric field strength during electroporation were studied in detail for one particular A. brasilense strain.