Open AccessCharacterisation of a degradative plasmid in Pseudomonas putida that controls the expression of 2,4‐xylenol degradative genes
Author(s) -
Dean H.F.,
Cheevadhanarak S.,
Skurray R.A.,
Bayly R.C.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03570.x
Subject(s) - pseudomonas putida , plasmid , gene , chemistry , pseudomonadales , pseudomonadaceae , strain (injury) , phenotype , pseudomonas , biochemistry , biology , genetics , bacteria , anatomy
Pseudomonas putida strain NCIB9866 (RA4000) carries an 85‐kb plasmid, pRA4000, can utilize p ‐cresol and 2,4‐xylenol, and is resistant to inorganic mercuric ions. The loss of this phenotype from a pRA4000‐cured derivative, combined with DNA analysis and conjugation studies, showed that pRA4000 encodes the degradation of p ‐cresol and resistance to inorganic mercuric ions. Studies using phenotypic variants of RA4000 altered in their ability to degrade 2,4‐xylenol and p ‐cresol indicated that structural genes for 2,4‐xylenol utilization are located on the chromosome and that their expression requires a positive regulator encoded on pRA4000.