Open AccessStudies on two major contaminating proteins of the cytoplasmic inclusion bodies in Escherichia coli
Author(s) -
Veeraragavan Kannappan
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03569.x
Subject(s) - escherichia coli , inclusion bodies , bacterial outer membrane , fusion protein , cytoplasm , recombinant dna , peptide sequence , enterobacteriaceae , biology , membrane protein , biochemistry , sequence (biology) , lac operon , chemistry , membrane , gene
Two major contaminating proteins of the cytoplasmic inclusion bodies in recombinant Escherichia coli overexpressing a LacZ‐papain fusion protein were studied. The proteins were isolated and their amino acid compostion and N‐terminal sequence analyses were performed. The N‐terminal sequence of these proteins were then compared with the sequence of E. coli proteins available in the data bank. The sequences of the contaminating proteins matched with the outer membrane protein II and outer membrane protein F of E. coli .