Open AccessStreptomyces cloning vector derived from Streptomyces lavendulae‐grasserius mini‐plasmid pSLG33
Author(s) -
Petřiček M.,
Tichý P.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03562.x
Subject(s) - plasmid , thiostrepton , biology , streptomyces , cloning vector , neomycin , t dna binary system , streptomycetaceae , molecular cloning , gene , multiple cloning site , genetics , microbiology and biotechnology , vector (molecular biology) , actinomycetales , bacteria , recombinant dna , gene expression , rna , antibiotics , ribosome
The cryptic multicopy plasmid designated pSLG33 (2.65 kb) was isolated from the vegetative mycelium of Streptomyces lavendulae‐grasserius RIA 746 and physically characterized. pRS410 vector (5.4 kb) was constructed by insertion of aph and tsr genes coding for neomycin and thiostrepton resistance, respectively, in a non‐essential part of the plasmid molecule. The pRS410 is compatible with multicopy Streptomyces plasmid vectors derived from pIJ101 plasmid.