Open AccessElectroporation induced transformation of Bacteroides ruminicola and Bacteroides uniformis by plasmid DNA
Author(s) -
Thomson Andrew M.,
Flint Harry J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03560.x
Subject(s) - electroporation , plasmid , bacteroides , transformation (genetics) , dna , shuttle vector , microbiology and biotechnology , biology , tetracycline , bacteria , genetics , recombinant dna , gene , antibiotics , vector (molecular biology)
Frequencies of greater than 10 5 transformants per μg DNA were achieved in Bacteroides ruminicola F101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid pRRI4. Similar procedures gave frequences of 10 6 erythromycin resistant transformants per μg DNA with the shuttle plasmid pDP1 (19 kbp) in Bacteroides uniformis . Transformation of B. uniformis occurred at a far lower frequency (10 3 μg) when pDP1 DNA was derived from E. coli rather than B. uniformis .