Open AccessAerobic and anaerobic metabolism in a facultative anaerobe Ep01 lacking cytochrome, quinone and catalase
Author(s) -
Niimura Youichi,
Koh Enki,
Uchimura Tai,
Ohara Naohiro,
Kozaki Michio
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03556.x
Subject(s) - biochemistry , acetate kinase , anaerobic exercise , catalase , formate , pyruvate dehydrogenase complex , pyruvate decarboxylation , chemistry , alcohol dehydrogenase , pyruvate dehydrogenase kinase , pyruvic acid , pyruvate decarboxylase , metabolic pathway , acetaldehyde , metabolism , ethanol , biology , enzyme , escherichia coli , physiology , gene , catalysis
A facultative anaerobe, strain Ep01 produced a mixture of pyruvate, formate, acetate and ethanol from glucose anaerobically, and acetate and pyruvate aerobically. Cell extract of anaerobic‐grown cells contained active pyruvate formatelyase, aldehyde dehydrogenase and alcohol dehydrogenase, while cell extract of aerobic grown cells contained an active pyruvate dehydrogenase system, NaDH oxidase and NADH peroxidase. Levels of acetate kinase and phosphate acetyltransferase activities were not significantly different in cells grown under either condition. Based on the metabolic products and the emzyme activities, we propose the presence of two metabolic pathways in strain Ep01, namely, a pathway to form formate, acetate and ethanol under anaerobic conditions, and a pathway to form under aerobic conditions. This explains why strain Ep01 can grow well both under strictly anaerobic conditions and well‐aerated conditions.