Open AccessCloning and sequencing of the nifH gene of Desulfovibrio gigas
Author(s) -
Kent Helen M.,
Buck Martin,
Evans David J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03555.x
Subject(s) - gene , genetics , open reading frame , biology , cloning (programming) , nucleic acid sequence , promoter , phylogenetic tree , base pair , peptide sequence , gene expression , computer science , programming language
The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open‐reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA ‐dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA .