Open AccessProtoporphyrinogen oxidation coupled to nitrite reduction with membranes from Desulfovibrio gigas
Author(s) -
Klemm Dwight J.,
Barton Larry L.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03553.x
Subject(s) - protoporphyrinogen oxidase , chemistry , nitrite , nitrite reductase , desulfovibrio , electron acceptor , photochemistry , biochemistry , organic chemistry , nitrate , sulfate , enzyme
The oxidation of protoporphyrinogen by membranes from Desulfovibrio gigas with nitrite as the electron acceptor proceeds at the ratio of 1 mol protoporphyrin produced for each mol of nitrite reduced. Coupled to the protoporphyrinogen‐nitrite reaction is the esterification of ortho‐phosphate with a P/2e − value of 0.92. Pentachlorophenol, 3 × 10 −5 M, and carbonyl cyanide m ‐chlorophenyl hydrazone, 3 × 10 −6 M, serve as uncouplers of phosphorylation while rotenone, 9 × 10 −6 M, and hydroxyquinoline‐ N ‐oxide, 0.1 × 10 −6 M, inhibit electron flow from protoporphyrinogen oxidase to nitrite reductase.