Open AccessMolecular cloning of an ice nucleation gene from Erwinia ananas and its expression in Escherichia coli
Author(s) -
Arai Soichi,
Abe Keiko,
Watabe Satoshi,
Emori Yasufumi,
Watanabe Michiko
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03551.x
Subject(s) - ananas , ice nucleus , escherichia coli , gene , microbiology and biotechnology , genomic dna , molecular cloning , biology , dna , strain (injury) , cloning (programming) , nucleation , genetics , chemistry , gene expression , botany , organic chemistry , anatomy , computer science , programming language
An approximately 7 kbp genomic DNA fragment was cloned from an ice nucleation‐active (ina) strain of Erwinia ananas and defined as to its restriction enzyme site. When the DNA fragment was introduced into E. coli MM294, a potent ice nucleation activity was expressed. Both 0.7 kbp truncation from the 5′‐end and 1.7 kbp truncation from the 3′‐end were also effective in expressing the ice nucleation activity in E. coli . Therefore, the resulting DNA fragment of approximately 5 kbp was considered to be an ina gene and named ina A. It existed as a unique gene in this strain of E. ananas . No corresponding ina gene existed in an ice nucleation‐inactive strain of E. milletiae .