Open AccessA simple procedure for detecting proteins that bind preferentially to kDNA networks
Author(s) -
Tittawella Ivor
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03499.x
Subject(s) - crithidia fasciculata , kinetoplast , biology , dna binding protein , dna , microbiology and biotechnology , biochemistry , gene , transcription factor
A procedure is described in which a fractionated protein extract from the trypanosome Crithidia fasciculata was incubated either with deproteinized C. fasciculata kDNA networks or nuclear DNA. At least three proteins in the range of 45–65 kDa had a specific affinity for the networks. We suggest that these proteins may play an important role in the biology of the kinetoplast.