Open AccessTransient exposure to a physiologically‐relevant concentration of calcium confers tobramycin resistance upon sessile cells of Pseudomonas aeruginosa
Author(s) -
Hoyle B.D.,
Costerton J.W.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03497.x
Subject(s) - tobramycin , pseudomonas aeruginosa , microbiology and biotechnology , biofilm , bacteria , pseudomonas , chemistry , antibiotics , pseudomonadaceae , calcium , biology , genetics , organic chemistry
Sessile populations of a mucoid Pseudomonas aeruginosa isolate generated in M‐56 medium of ‘low’ (0.02 mM) or ‘high’ (2.5 mM) Ca 2+ were treated with tobramycin (250 μg·ml −1 ), as were biofilms exposed transiently to ‘high’ Ca 2+ medium. Viability decreased by over 99.9% within 8 h in the ‘low’ and ‘high’ samples, while transient Ca 2+ exposure was protective. Dispersed sessile bacteria were as tobramycin sensitive as planktonic bacteria.