Open AccessIdentification of a repetitive DNA sequence specific to Mycobacterium paratuberculosis
Author(s) -
Collins Desmond M.,
Gabric Diana M.,
Lisle Geoffrey W.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03440.x
Subject(s) - paratuberculosis , biology , microbiology and biotechnology , serotype , mycobacterium , sequence analysis , virology , whole genome sequencing , mycobacterium avium subsp. paratuberculosis , dna sequencing , dna , sequence (biology) , genome , genetics , bacteria , gene
A 0.2‐kb DNA sequence specific to Mycobacterium paratuberculosis , the causative organism of Johne's disease, was isolated from a partial genomic library. The sequence was part of a larger repetitive DNA element and was present in strains of M. paratuberculosis from cattle, sheep, goat, deer and also a woman with Crohn's disease but not in M. paratuberculosis strain 18. The sequence was not present in strains of 19 other mycobacterial species including 31 reference serotype strains of the M. avium‐M. intracellular‐M. scrofulaceum (MAIS) complex, some strains of which are closely related to M. paratuberculosis . The sequence may be useful for developing a diagnostic test for Johne's disease.