Open AccessAn NADH: Fe(III) EDTA oxidoreductase from Cryptococcus albidus : an enzyme involved in ethylene production in vivo?
Author(s) -
Fukuda Hideo,
Takahashi Masaya,
Fujii Takao,
Tazaki Masato,
Ogawa Takahira
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03428.x
Subject(s) - ethylene , oxidoreductase , nad+ kinase , chemistry , enzyme , chelation , nuclear chemistry , medicinal chemistry , biochemistry , inorganic chemistry , catalysis
An ethylene‐forming enzyme which forms ethylene from 2‐oxo‐4‐methylthiobutyric acid (KMBA) was purified to an electrophoretically homogeneous state from a cell‐free extract of Cryptococcus albidus IFP 0939. The presence of KMBA, NADH, Fe(III) chelated to EDTA and oxygen were essential for the formation of ethylene. When ferric ions, as Fe(III)EDTA, in the reaction mixture were replaced by Fe(II)EDTA under aerobic conditions, the non‐enzymatic formation of ethylene was observed. Under anaerobic conditions in the presence of Fe(III)EDTA and NADH, the enzyme reduced 2 mol of Fe(III) with 1 mol of NADH to give 2 mol of Fe(II) and 1 mol NAD + , indicating that the ethylene‐forming enzyme is an NADH‐Fe(III)EDTA oxidoreductase. The role of NADH:Fe(III)EDTA oxidoreductase activity in the formation in vivo ethylene from KMBA is discussed.