Open AccessCalmodulin in heterocystous cyanobacteria: biochemical and immunological evidence
Author(s) -
Pettersson Annette,
Bergman Birgitta
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03426.x
Subject(s) - cyanobacteria , calmodulin , heterocyst , immunogold labelling , egta , anabaena , biology , biochemistry , cytoplasm , western blot , antiserum , botany , bacteria , calcium , chemistry , enzyme , ultrastructure , antibody , immunology , genetics , organic chemistry , gene
The occurrence activity and localization of calmodulin in three heterocystous cyanobacteria of the genus Anabaena were studied. Boiled crude extracts caused a Ca 2+ ‐dependent stimulation of NAD kinase. Such a stimulation was blocked by EGTA and chlorpromazine. SDS‐PAGE and Western blot analysis using antiserum against eukaryotic spinach calmodulin, revealed a polypeptide of about 17 kDa. Immunogold localization of calmodulin gave a dense gold label both vegetative cells and heterocysts. The label was mainly confined to the centroplasm in vegetative cells, while it was evenly distributed in the cytoplasm of mature heterocysts.