Open AccessComparison of R‐ and S‐form lipopolysaccharides fractionated from Escherichia coli UKT‐B lipopolysaccharide in pyrogen and Limulus tests
Author(s) -
Komuro Tetsuo,
Yomota Chikako,
Kimura Toshio,
Galanos Chris
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03423.x
Subject(s) - limulus , lipopolysaccharide , escherichia coli , polyacrylamide gel electrophoresis , chemistry , chromatography , sodium , microbiology and biotechnology , biology , biochemistry , immunology , enzyme , organic chemistry , paleontology , gene
Different LPS was shown to have a relatively different proportion of O‐specific chain‐less (R‐form) LPS by polyacrylamide gel electrophoresis (PAGE) with sodium deoxycholate (DOC). By using DOC‐PAGE, S‐form LPS having O‐chain with approximately 11 repeating units on average (S‐Fr) and O‐chain‐less LPS (R‐Fr) were separated from Escherichia coli UKT‐B S‐form LPS. Significantly stronger pyrogenicity was observed in R‐Fr than in S‐Fr when measured on the weight basis. Similar result was observed in Limulus test. Comparing biological activities of different S‐form LPS, attention should be given to the amounts of co‐existing R‐form LPS.