Open AccessThe ATP‐dependent synthesis of factor 390 by cell‐free extracts of Methanobacterium thermoautotrophicum (strain ΔH)
Author(s) -
Kengen ServéW.M.,
Keltjens Jan T.,
Vogels Godfried D.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03409.x
Subject(s) - methanobacterium , gtp' , cofactor , biochemistry , pyrophosphate , cell free system , enzyme , atp synthase , strain (injury) , chemistry , adenosine triphosphate , coenzyme a , stereochemistry , biology , reductase , archaea , gene , anatomy
Cell‐free extracts of Methanobacterium thermoautotrophicum (strain ΔH) converted the 8‐OH‐5‐deazaflavin coenzyme F 420 to factor 390, a 8‐adenylyl derivative (F 420 ‐AMP). Activity was only observed upon exposure of the crude cell‐free extract to oxygen. The ability to synthesize F 390 was lost when crude cell‐free extract was subsequently brought to an anaerobic reducing environment. The enzymatic reaction used ATP and oxidized coenzyme F 420 as substrates and inorganic pyrophosphate was formed next to F 390 . GTP could be used instead of ATP resulting in a guanylylated derivative. The crude cell‐free extract showed K m values of 154 μM for coenzyme F 420 and 2.4 mM for ATP. A partially purified enzyme preparation exhibited a K eq of 0.32. In accordance, coenzyme F 420 and ATP could be synthesized from F 390 and PPi by the reverse reaction.