Open AccessCholine and betaine as inducer agents of Pseudomonas aeruginosa phospholipase C activity in high phosphate medium
Author(s) -
Lucchesi Gloria I.,
Lisa Teresita A.,
Domenech Carlos E.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03359.x
Subject(s) - betaine , pseudomonas aeruginosa , choline , phosphate , phospholipase c , inducer , alkaline phosphatase , phospholipase , biochemistry , phosphatase , enzyme , phospholipase d , pseudomonas , acid phosphatase , pseudomonadales , chemistry , glycine , microbiology and biotechnology , biology , bacteria , amino acid , genetics , gene
In Pseudomonas aeruginosa , choline or betaine employed as the sole carbon and nitrogen source in a high phosphate medium induced a phopholipase C and an acid phosphatase activity but not an alkaline phosphatase activity. The P. aeruginosa strain utilized in this work does not possess a constitutive phospholipase C, since under culture conditions identical to those utilized by other authors (J. Bacteriol. 93, 670–674 (1967) and J. Bacteriol. 150, 730–738 (1982)), our phospholipase C proved to be an inorganic phosphate‐repressible enzyme. These findings enable us to conclude that although the phosphate control for the synthesis of phospholipase C may exist, it is expressed only under certain favorable culture conditions.