Open AccessCloning and expression in Escherichia coli of the toluene dioxygenase gene from Pseudomonas putida NCIB11767
Author(s) -
Stephens Gillian M.,
Sidebotham Julie M.,
Mann Nicholas H.,
Dalton Howard
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03352.x
Subject(s) - pseudomonas putida , escherichia coli , dioxygenase , molecular cloning , toluene , chemistry , biochemistry , gene , microbiology and biotechnology , pseudomonas , biology , recombinant dna , gene expression , bacteria , genetics , organic chemistry
The genes encoding toluene dioxygenase, toluene cis ‐glycol dehydrogenase and catechol 2,3‐oxygenase from Pseudomonas putida NCIB 11767 were cloned and expressed in Escherichia coli HB101 on a 20 kb fragment. The recombinant strain produced indigo and a variety of other coloured products. Although the enzymes were expressed in the absence of inducers, further induction was observed in the presence of toluene or benzene, implying the presence of regulatory elements on the 20 kb insert.