Open AccessProduction, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b
Author(s) -
Kreft Jürgen,
Funke Dorothee,
Haas Albert,
Lottspeich Friedrich,
Goebel Werner
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03298.x
Subject(s) - listeriolysin o , listeria monocytogenes , hemolysin , cytolysin , listeria , microbiology and biotechnology , hemolysis , biology , chemistry , biochemistry , bacteria , virulence , gene , genetics , immunology
In culture supernatants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH‐activated cytolysin, and which was therefore identified as listeriolysin O (LLO). In the case of L. ivanovii a second major supernatant protein of molecular weight 24 kDa co‐purified with LLO. However, the function of this protein has to be determined. In culture supernatants of L. ivanovii a sphingomyelinase and a lecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii . The N‐terminal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.