Open AccessImmunological detection of penicillin‐binding protein 2′ in clinical isolates of methicillin‐resistant Staphylococcus aureus and Staphylococcus epidermidis
Author(s) -
O'Hara Denise M.,
Harrington Charles R.,
Reynolds Peter E.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03228.x
Subject(s) - microbiology and biotechnology , staphylococcus aureus , penicillin binding proteins , staphylococcus epidermidis , penicillin , staphylococcus haemolyticus , micrococcaceae , biology , staphylococcus , meticillin , staphylococcal infections , antibiotics , bacteria , virology , antibacterial agent , genetics
The additional penicillin‐binding protein (PBP 2′) that is important in determining intrinsic resistance in methicillin‐resistant strains of Staphylococcus aureus (MRSA) has been detected immunologically in strains from a variety of world‐wide locations. This additional protein has also been definitively identified both immunologically and as a PBP in methicillin‐resistant strains of S. epidermidis (MRSE). The assay described is rapid, specific and sensitive and has been used to detect PBP 2′ in S. haemolyticus but not in β‐lactam resistant Streptococci.