Open AccessThe complete nucleotide sequence of the glutamine synthetase gene ( glnA ) of Bacillus subtilis
Author(s) -
Nakano Yoshio,
Tanaka Eiichi,
Kato Chiaki,
Kimura Kinuko,
Horikoshi Koki
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03225.x
Subject(s) - bacillus subtilis , nucleic acid sequence , clostridium acetobutylicum , shuttle vector , glutamine synthetase , biology , microbiology and biotechnology , gene , plasmid , homology (biology) , peptide sequence , escherichia coli , open reading frame , amino acid , biochemistry , genetics , glutamine , recombinant dna , bacteria , vector (molecular biology) , butanol , ethanol
The glutamine synthetase (GS) gene from Bacillus subtilis PCI 219 was cloned in Escherichia coli using the vector pBR329. A plasmid, pSGS2, was isolated from a glnA + transformant and the cloned GS gene was found to be located in a 3.6 kb DNA fragment. The nucleotide sequence of a 1.8 kb segment encoding the GS was determined. This segment showed an open reading frame which would encode a polypeptide of 444 amino acids. The amino acid sequence of this GS gene product has higher homology with that of the Clostridium acetobutylicum GS than that of the E. coli GS.