Open AccessCloning and characterization of the carbapenem biosynthetic genes from Streptomyces fulvoviridis
Author(s) -
Nakata Kuniho,
Horinouchi Sueharu,
Beppu Teruhiko
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03219.x
Subject(s) - streptomyces griseus , mutant , plasmid , cloning (programming) , biology , gene , streptomyces , southern blot , carbapenem , genetics , molecular cloning , microbiology and biotechnology , peptide sequence , bacteria , computer science , programming language , antibiotics
Carbapenen non‐producing mutants were isolated from Streptomyces fulvoviridis and divided into six cosynthesis groups. By using one of the mutants as the host and plasmid pIJ385 as the vector, we cloned carbapenem biosynthetic genes from the parental S. fulvoviridis strain. A cloned 6‐kb DNA fragment complemented the defects of three mutants each of which had a mutation in different genes. Southern blot hybridization using the cloned 6‐kb fragment as probe showed the presence of the nucleotide sequences homologous to the probe in other carbapenem‐producing Streptomyces spp. In addition, Streptomyces griseus , a carbapenem non‐producer, possessed the sequence homologous to the probe and showed co‐synthesis phenomena with some of the carbapenem non‐producing mutants of S. fulvoviridis .