Open AccessEffects of 2‐deoxyglucose on Saccharomyces cerevisiae as observed by in vivo 31 P‐NMR
Author(s) -
LoureiroDias Maria C.,
Santos Helena
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03214.x
Subject(s) - deoxyglucose , saccharomyces cerevisiae , chemistry , polyphosphate , in vivo , incubation , biochemistry , nuclear magnetic resonance spectroscopy , yeast , biology , stereochemistry , phosphate , microbiology and biotechnology
Saccharomyces cerevisiae cells were treated with 2‐deoxyglucose (1 mM) and the effects induced in the levels of phosphorus compounds and in the internal pH were monitored using 31 P‐NMR. Upon incubation with 2‐deoxyglucose a strong decrease in the polyphosphate level was observed and the cytoplasmic pH decreased by about 0.4 units. This shows that 2‐deoxyglucose strongly interferes with the cell conditions and consequently, the results of experiments in which 2‐deoxyglucose was used to obtain deenergized cells should be carefully reanalysed.