Open AccessEnergy‐dependent uptake of urea by Bacillus megaterium
Author(s) -
Jahns Thomas,
Kaltwasser Heinrich
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03212.x
Subject(s) - bacillus megaterium , urea , urease , nitrogen , chemistry , biochemistry , in vivo , urea cycle , in vitro , biology , nuclear chemistry , bacteria , amino acid , organic chemistry , arginine , microbiology and biotechnology , genetics
Evidence for the existence of an energy‐dependent urea uptake system in Bacillus megaterium DSM 90 was obtained by studying the uptake of 14 C‐urea. In vivo urea uptake and in vitro urease activity differed significantly with respect to temperature‐ and pH‐dependence, kinetic parameters and response towards metabolic inhibitors. Highest uptake activities were observed during exponential growth, and a rapid decrease in urea uptake occurred when cells entered the stationary growth phase and started to sporulate. Significant differences in the uptake rates were observed during growth with different nitrogen sources, suggesting that the formation of the system is under nitrogen control.