Open AccessDetection and identification of pathotypes of verocytotoxigenic Escherichia coli isolated from weaned piglets using gene probes for seven E. coli toxins
Author(s) -
Mainil J.,
Daube G.,
Deprez P.,
Kaeckenbeeck A.,
Pohl P.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03136.x
Subject(s) - escherichia coli , biology , identification (biology) , microbiology and biotechnology , enterobacteriaceae , gene , escherichia coli proteins , genetics , botany
Abstract Seventy verocytotoxigenic (VTEC) and sixtythree non VTEC haemolytic Escherichia coli isolated from recently weaned piglets were examined by the colony hybridization assay using gene probes for three verocytotoxins: Edema disease principle (EDP) and Shiga‐like toxins I and II (SLTI and SLTII). The results with the EDP and SLTII probes were identical. All VTEC hybridized with these two probes, while non VTEC did not. All 133 E. coli were negative for the SLTI probe. Hybridization of the plasmid content of 14 VTEC did not show any evidence for plasmid localization of the genes coding for the EDP. The 70 VTEC were also assayed with gene probes for heat‐stable (STaP, STb) and heat‐labile (LT, LTIIa) enterotoxins. Only the STb probe was hybridized by 36 of them. Most STb‐positive isolates belonged to serotype O141: K85 biotypes 9 and 13 PC.