Open AccesspH dependence of uptake hydrogenases in Azospirillum brasilense and A. amazonense
Author(s) -
Tito Donald,
Knowles Roger
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03134.x
Subject(s) - hydrogenase , methylene blue , azospirillum brasilense , in vivo , in vitro , chemistry , biochemistry , bacteria , enzyme , biology , catalysis , microbial inoculant , microbiology and biotechnology , photocatalysis , genetics
Methylene blue (MB)‐dependent uptake hydrogenase activity of Azospirillum brasilense was at least 30% higher than O 2 ‐dependent activity in this species, whereas for A. amazonense the reverse was true. Both MB‐dependent and O 2 ‐dependent uptake hydrogenase activities of A. brasilense showed maxima at pH 7.0 both in vivo and in vitro. For A. amazonense , both in vivo and in vitro, the MB‐dependent uptake hydrogenase activity increased roughly linearly as pH values were increased from pH 6.25 to 7.75, whereas the O 2 ‐dependent activity was almost unaffected by pH. The optimal pH for A. amazonense growth was broad and in the range of 5.4 to 6.5.