Open AccessIron depletion alters surface‐associated properties of Staphylococcus aureus and its association to human neutrophils in chemiluminescence
Author(s) -
Domingue P.A.G.,
Lambert P.A.,
Brown M.R.W.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03122.x
Subject(s) - staphylococcus aureus , phagocytosis , chemiluminescence , microbiology and biotechnology , chemistry , antigen , strain (injury) , in vivo , phenotype , bacteria , biology , biochemistry , immunology , chromatography , gene , genetics , anatomy
Staphylococcus aureus NCTC 6571 was grown in iron‐depleted tryptone soya broth (Fe ‐ TSB) to approximate to in vivo conditions, and in iron‐rich TSB (Fe + TSB). Low iron effected a crucial decrease in surface hydrophobicity (SH) and a lack of supernatant Protein A (PrA). Iron availability did not affect PrA detection in immunoblotting and it was identified as a 35.5 kDa antigen in this strain. Fe‐phenotypes lacked 34, 48 and 52 kDa antigens. In chemiluminescence, Fe‐phenotypes appeared least vulnerable to phagocytosis despite opsonisation.