Open AccessSpectrophotometric identification of 8‐hydroxy‐5‐deazaflavin: NADPH oxidoreductase activity in streptomycetes producing tetracyclines
Author(s) -
Novotná Jana,
Neužil Jiří,
Hoš?álek Zdeněk
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03118.x
Subject(s) - streptomyces aureofaciens , streptomyces , actinomycetales , spore , mycelium , streptomycetaceae , oxidoreductase , biochemistry , biology , microbiology and biotechnology , substrate (aquarium) , reductase , chemistry , enzyme , bacteria , botany , ecology , genetics
Cell‐free extracts of vegetative mycelia of Streptomyces aureofaciens and Streptomyces rimosus were found to reduce streptomycete‐origin 8‐hydroxy‐5‐deazaisoalloxazine derivatives (SF 420 ) using NADPH as a dnor of hydrogen and electrons. 7,8‐didemethyl‐8‐hydroxy‐5‐deazariboflavin (F 0 ) also was a substrate, although with a lower reaction rate than that for SF 420 . NADH could not substitute for NADPH. The F 420 ‐reductase activity was also observed in homogenates of S. aureofaciens spores.