Open AccessCloning and expression of an entomocidal protein gene from Bacillus thuringiensis galleriae toxic to both lepidoptera and diptera
Author(s) -
Ahmad W.,
Nicholls C.,
Ellar D.J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03109.x
Subject(s) - bacillus thuringiensis , shuttle vector , bacillus megaterium , microbiology and biotechnology , biology , plasmid , bacillus subtilis , escherichia coli , gene , lepidoptera genitalia , molecular cloning , antiserum , dna , gene expression , recombinant dna , bacteria , vector (molecular biology) , genetics , botany , antibody
A gene encoding a 61 kDa entomocidal (P2) protein from Bacillus thuringiensis galleriae was cloned in Escherichia coli using oligonucleotide probes corresponding to N‐ and C‐terminal DNA sequences of a Kurstaki P2 gene. When the gene of a 5.8 kb Hin dIII fragment was transformed into B. subtilis on a shuttle vector, sporulation was completely inhibited and expression could not be detected. When B. megaterium was transformed with the same plasmid, only 10% of the cells sporulated and a 61 kDa P2 protein which cross‐reacted with kurstaki P2 antiserum was synthesised. Cell lysates of the transformed B. megaterium were found to be toxic to both lepidopteran and dipteran larvae.