Open AccessEvolution of Pseudomonas aeruginosa cells towards a filterable stage in seawater
Author(s) -
Bakhrouf Amina,
Jeddi Moncef,
Bouddabous Abdellatif,
Gauthier Michel J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03107.x
Subject(s) - seawater , pseudomonas aeruginosa , microbiology and biotechnology , bacteria , biology , pseudomonas , artificial seawater , urease , pseudomonadaceae , chemistry , enzyme , biochemistry , ecology , genetics
In sterile nutrient‐free seawater, the number of Pseudomonas aeruginosa culturable cells decreased progressively over time and the bacteria developed cells capable of passing through a 0.45 μm pore membrane. This development was more rapid in non‐autoclaved, stirred seawater and the recovery of filterable cells varied depending on the membrane type used. Minicells were observed under an electron microscope. They yielded normal cells in bacteriological media with analogous colonies and an unchanged antibiotic resistance profile. Some biochemical characters, such as gelatinase or urease activity, were however modified in the filterable cells.