Open AccessCloning and expression of a Clostridium acetobutylicum α‐amylase gene in Escherichia coli
Author(s) -
Verhasselt Peter,
Poncelet Filip,
Vits Karel,
Van Gool August,
Vanderleyden Jos
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03097.x
Subject(s) - clostridium acetobutylicum , escherichia coli , cloning (programming) , microbiology and biotechnology , clostridium , gene , gene expression , biology , amylase , molecular cloning , bacteria , chemistry , genetics , biochemistry , enzyme , ethanol , butanol , computer science , programming language
A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzin clones. One clone in which the recombinant plasmid, pVP101, conferred α‐amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45‐kbp Bgl II restriction fragment. A detailed physical map of pVP101 is presented.