Open AccessGrowth characteristics of Tyzzer's organism in cultured mouse hepatocytes
Author(s) -
Kawamura Seiji,
Mitsuoka Tomotari,
Fujiwara Kosaku
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03095.x
Subject(s) - titer , incubation , biology , microbiology and biotechnology , bacteria , incubation period , immunofluorescence , bacterial growth , virology , antibody , immunology , biochemistry , genetics , virus
The growth characteristics of Tyzzer's organisms in cultured mouse hepatocytes were examined by direct bacterial counting and plaque assay. The viability of propagated bacteria and time required for the maximum titers depended on incubation time and inoculum dose, respectively. Both infectious titers and bacterial counts at optimum harvests were much higher than those obtained from infected mouse livers. Immunofluorescence revealed, after a lag phase of about 2 h, a linear multiplication during a 6 h period.